EGFP-CAAX | Addgene 86056 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

EGFP-CAAX

catalog :

86056

citations: 6

Reference
Mandal T, Biswas A, Ghosh T, Manikandan S, Kundu A, Banerjee A, et al. Mechano-regulation by clathrin pit-formation and passive cholesterol-dependent tubules during de-adhesion. Cell Mol Life Sci. 2024;81:43 pubmed publisher
de Boer L, Vanes L, Melgrati S, Biggs O May J, Hayward D, Driscoll P, et al. T cell migration requires ion and water influx to regulate actin polymerization. Nat Commun. 2023;14:7844 pubmed publisher
Duque M, Lee Kubli C, Tufail Y, Magaram U, Patel J, Chakraborty A, et al. Sonogenetic control of mammalian cells using exogenous Transient Receptor Potential A1 channels. Nat Commun. 2022;13:600 pubmed publisher
Adams G, López M, Cartagena Rivera A, Waterman C. Survey of cancer cell anatomy in non-adhesive confinement reveals a role for filamin-A and fascin-1 in leader bleb-based migration. Mol Biol Cell. 2021;:mbcE21040174 pubmed publisher
Cabral Dias R, Awadeh Y, Botelho R, Antonescu C. Detection of Plasma Membrane Phosphoinositide Dynamics Using Genetically Encoded Fluorescent Protein Probes. Methods Mol Biol. 2021;2251:73-89 pubmed publisher
Madugula V, Lu L. A ternary complex comprising transportin1, Rab8 and the ciliary targeting signal directs proteins to ciliary membranes. J Cell Sci. 2016;129:3922-3934 pubmed

product information

Catalog Number :

86056

Product Name :

EGFP-CAAX

article :

doi	10.1242/jcs.194019
id	22774
pubmed_id	27633000

bacterial resistance :

Kanamycin

cloning :

backbone

pEGFP-C1

backbone_mutation

backbone_origin

Clontech

backbone_size

4700

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth strain :

A mammalian expression plasmid encoding farnesylation motif of HRAS with N-terminus EGFP.

origin :

pi :

alt_names

cloning

clone_method	Restriction Enzyme
cloning_site_3	BamHI
cloning_site_5	EcoRI
promoter	CMV
sequencing_primer_3
sequencing_primer_5	5' TTTAGTGAACCGTCAGATC 3'
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	C-BAS/HAS, C-H-RAS, C-HA-RAS1, CTLO, H-RASIDX, HAMSV, HRAS1, RASH1, p21ras
gene	HRAS
id	3265

genbank_ids

NM_001318054.1

mutation

contains the region from 825-857 of HRAS as a farnesylation motif

name

Homo sapiens Harvey rat sarcoma

shRNA_sequence

size

species

9606

Homo sapiens