This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
GST-thr-RAF1-RBD
catalog :
86033
citations: 1
Reference
Haza K, Martin H, Rao A, Turner A, Saunders S, Petersen B, et al. RAS-inhibiting biologics identify and probe druggable pockets including an SII-α3 allosteric site. Nat Commun. 2021;12:4045 pubmed publisher
product information
Catalog Number :
86033
Product Name :
GST-thr-RAF1-RBD
article :
doi
id22824
pubmed_id
bacterial resistance :
Ampicillin
cloning :
backbonepDest-521
backbone_mutationDerivative of pET43 modified for Gateway recombinational cloning
backbone_originin-house
backbone_size5516
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth notes :
modified from a plasmid described originally in Taylor and Shalloway (1996), Current Biology 6(12): 1621-27. This construct contains an E. coli optimized DNA sequence to improve expression and Gateway elements for recombinational cloning.
growth strain :
Expresses the RAS-binding domain of RAF1 for in vitro analysis of RAS activation
origin :
37
pi :
alt_names
cloning
clone_methodGateway Cloning
cloning_site_3
cloning_site_5
promoterT7
sequencing_primer_3ggtggtgctcgagatcctctggg
sequencing_primer_5TTAATACGACTCACTATAGGGG
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesCMD1NN, CRAF, NS5, Raf-1, c-Raf
geneRAF1
id5894
genbank_ids
mutationContains residues 1-149
nameRAF1 RBD (Ras-binding domain)
shRNA_sequence
size1154
species
9606
Homo sapiens
tags
locationN terminal on insert
tagGST
resistance markers :
139
tags :
Low Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA