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product summary
company name :
Addgene
product type :
cDNA
product name :
pQhsCDtRNA-
catalog :
86015
citations: 1
product information
Catalog Number :
86015
Product Name :
pQhsCDtRNA-
article :
| doi | PONE-D-14-15013 [pii] |
| id | 22820 |
| pubmed_id | 25078083 |
bacterial resistance :
Ampicillin
cloning :
| backbone | pQLinkN (Addgene #13670) | |||
| backbone_mutation | ||||
| backbone_origin | Konrad Buessow lab | |||
| backbone_size | ||||
| promoter | ||||
| sequencing_primer_3 | ||||
| sequencing_primer_5 | ||||
| vector_types |
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growth notes :
Please see associated publication for additional graphics describing this plasmid. Plasmid construction: To construct the human box C/D plasmid, pQhsCD, pQLinkN (Addgene #13670) was inserted with the coding sequences of Homo sapiens (Hs) NOP56 (amino acids 1 to 411), NOP58 (amino acids 1 to 401) and fibrillarin (amino acids 83 to 316), 15.5 K, and U14 snoRNA. This plasmid differs from #86014 in that U14 is not part of a tRNA-containing minigene. Each protein-encoding gene was cloned into pQLink-N plasmid using BamHI and NotI sites. The plasmids containing two different protein-encoding genes were then digested by SwaI and PacI, respectively, treated with LIC qualified T4 polymerase (dCTP for Pac I digest and dGTP for Swa I digest) and then annealed at 70 C. Each clone was identified by digestion of the recombined plasmid with Pac I. This process was repeated for additional coding sequence until all were inserted.
growth strain :
Multi-gene expression system with human box C/D proteins with U14
origin :
37
pi :
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resistance markers :
| 3184 |
tags :
Unknown
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
Watertown, MA 02472
info@addgene.org
https://www.addgene.org617.225.9000
headquarters: USA
questions and comments