This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
LVXN-Neo-NSD2
catalog :
86010
citations: 1
Reference
Jaffe J, Wang Y, Chan H, Zhang J, Huether R, Kryukov G, et al. Global chromatin profiling reveals NSD2 mutations in pediatric acute lymphoblastic leukemia. Nat Genet. 2013;45:1386-91 pubmed publisher
product information
Catalog Number :
86010
Product Name :
LVXN-Neo-NSD2
article :
doi10.1038/ng.2777
id22816
pubmed_id24076604
bacterial resistance :
Ampicillin
cloning :
backboneUTF-8'en-us'pLVX-IRES-Neo_DS_SEQ
backbone_mutation
backbone_originClontech
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
Lentiviral
growth notes :
Please note that mutation K1002R was found during Addgene's quality control. The depositor noted that this mutation does NOT affect plasmid function.
growth strain :
express wild-type NSD2 in mammalian cells
growth temp :
Since this plasmid is prone to recombination, we recommend testing 2-4 colonies to ensure the full plasmid is intact.
origin :
30
pi :
alt_names
cloning
clone_methodGateway Cloning
cloning_site_3
cloning_site_5
promoterCMV
sequencing_primer_3aggtgtatcttatacacgt
sequencing_primer_5taggcgtgtacggtgggagg
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesKMT3F, KMT3G, MMSET, RAUST, REIIBP, TRX5, WHS, WHSC1
geneNSD2
id7468
genbank_ids
NM_001042424
mutationK1002R (please see depositor comments below)
nameNSD2
shRNA_sequence
size4098
species
9606
Homo sapiens
tags
locationN terminal on insert
tagFlag tag D Y K D D D D K
plasmid copy :
GNF
resistance markers :
3154
tags :
Low Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA