This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pET28a SUMO Nup53xl 1-320
catalog :
85998
citations: 2
Reference
Mafi A, Purohit R, Vielmas E, Lauinger A, Lam B, Cheng Y, et al. Hedgehog proteins create a dynamic cholesterol interface. PLoS ONE. 2021;16:e0246814 pubmed publisher
Vollmer B, Schooley A, Sachdev R, Eisenhardt N, Schneider A, Sieverding C, et al. Dimerization and direct membrane interaction of Nup53 contribute to nuclear pore complex assembly. EMBO J. 2012;31:4072-84 pubmed publisher
product information
Catalog Number :
85998
Product Name :
pET28a SUMO Nup53xl 1-320
article :
doi10.1038/emboj.2012.256
id22812
pubmed_id22960634
bacterial resistance :
Kanamycin
cloning :
backbonepET28a
backbone_mutationremoval of the N-Terminal HIS6 tag, addition of teh MISTIC sequence upstream of the MCS
backbone_originNovagene
backbone_size5300
promoterT7
sequencing_primer_3pETrv
sequencing_primer_5lac
vector_types
Bacterial Expression
growth strain :
bacterial expression of his6 and SUMO tagged Xenopus laevis Nup53
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3Xho
cloning_site_5Nde
promoter
sequencing_primer_3pEtrv
sequencing_primer_5pETfw
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesXELAEV_18044791mg, nup35, nup53
genenup35.L
id379446
genbank_ids
mutationcodon optimzed for bacterial expression
nameXenopus laevis Nup53
shRNA_sequence
size960
species
8355
Xenopus laevis
tags
locationN terminal on insert
tagSUMO
resistance markers :
3180
tags :
Low Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA