This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
CMV epArcLight
catalog :
85803
citations: 1
Reference
Platisa J, Vasan G, Yang A, Pieribone V. Directed Evolution of Key Residues in Fluorescent Protein Inverses the Polarity of Voltage Sensitivity in the Genetically Encoded Indicator ArcLight. ACS Chem Neurosci. 2017;8:513-523 pubmed publisher
product information
Catalog Number :
85803
Product Name :
CMV epArcLight
article :
doi10.1021/acschemneuro.6b00234
id23064
pubmed_id28045247
bacterial resistance :
Ampicillin
cloning :
backbonepCS2+ CMV
backbone_mutation
backbone_origin
backbone_size5694
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
A portion of this plasmid was derived from a plasmid made by Dr. Atsushi Miyawaki, Laboratory for Cell Function Dynamics, Brain Science Institute, RIKEN, 2-1 Hirosawa, Saitama 351-0198, Japan.
growth strain :
genetically-encoded fluorescent voltage indicator
origin :
37
pi :
alt_names
CiVSD-ecliptic pHluorin
CiSP
ecliptic pHluorin
cloning
clone_methodRestriction Enzyme
cloning_site_3XbaI
cloning_site_5HindIII
promoterCMV
sequencing_primer_3tgcgtgtggttcgattagcaag
sequencing_primer_5SP6
site_3_destroyed
site_5_destroyed
entrez_gene
genbank_ids
AB183035
AF058695.1
mutationCi-VSP contains R217Q mutation; ecliptic pHluorin contains A227D mutation. The A227D mutation increases the fluorescence response magnitude.
nameepArcLight
shRNA_sequence
size
species
32644
Other
Ciona intestinalis
tags
plasmid copy :
Dr. Atsushi Miyawaki, Laboratory for Cell Function Dynamics, Brain Science Institute, RIKEN, 2-1 Hirosawa, Saitama 351-0198, Japan.
resistance markers :
1258
tags :
High Copy
terms :
Hygromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA