This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pAAVS1-Neo-UBC-M2rtTA-H2BmNeon (donorAB-UBC-mNeon)
catalog :
85800
citations: 1
Reference
Tejero R, Huang Y, Katsyv I, Kluge M, Lin J, Tome Garcia J, et al. Gene signatures of quiescent glioblastoma cells reveal mesenchymal shift and interactions with niche microenvironment. EBioMedicine. 2019;42:252-269 pubmed publisher
product information
Catalog Number :
85800
Product Name :
pAAVS1-Neo-UBC-M2rtTA-H2BmNeon (donorAB-UBC-mNeon)
article :
doi10.1016/j.ebiom.2019.03.064
id22788
pubmed_id30952620
bacterial resistance :
Ampicillin
cloning :
backboneN/A
backbone_mutation
backbone_origin
backbone_size3961
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Other
Human Targeting
growth notes :
Plasmid cloned by combining vectors obtained from Hockemeyer and Jaenisch (Addgene #60843) laboratories
growth strain :
donorAB-UBC-Neon targeting vector for human AAVS1 locus; knock-in of Dox controlled H2Bneon
origin :
30
pi :
alt_names
AAVS1-homol arms T2A-Neo UBC-rtTA-M2 tetO-H2B-mNeon
donor AB-UBC-mNeon
cloning
clone_methodRestriction Enzyme
cloning_site_3RruI
cloning_site_5AjuI
promoter
sequencing_primer_3
sequencing_primer_5T3
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesAAVS1, LENG3, MBS85, p84, p85
genePPP1R12C
id54776
genbank_ids
mutationhomology arms for knock-in into first intron
namePPP1R12C
shRNA_sequence
size7358
species
9606
Homo sapiens
32644
Other
tags
resistance markers :
2483
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA