This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pEGFP-p97-E578Q
catalog :
85672
citations: 1
Reference
Papadopoulos C, Kirchner P, Bug M, Grum D, Koerver L, Schulze N, et al. VCP/p97 cooperates with YOD1, UBXD1 and PLAA to drive clearance of ruptured lysosomes by autophagy. EMBO J. 2017;36:135-150 pubmed publisher
product information
Catalog Number :
85672
Product Name :
pEGFP-p97-E578Q
article :
doi10.15252/embj.201695148
id22737
pubmed_id27753622
bacterial resistance :
Kanamycin
cloning :
backbonepEGFP-C1
backbone_mutation
backbone_origin
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
Addgene NGS results found the addition of amino acids GSTGSR at the C-terminus of the p97 translation
growth strain :
Expression of human p97 E578Q mutant with N-terminal GFP tag
origin :
37
pi :
alt_names
VCP
valosin-containing protein
cloning
clone_methodRestriction Enzyme
cloning_site_3XhoI
cloning_site_5BamHI
promoterCMV
sequencing_primer_3SV40pA-R
sequencing_primer_5CMV-F
site_3_destroyed
site_5_destroyed
entrez_gene
aliases
geneVcp
id116643
genbank_ids
mutationE578Q
namep97
shRNA_sequence
size
species
10116
Rattus norvegicus
tags
locationN terminal on backbone
tagGFP
plasmid copy :
cloned from pcDNA5-p97-E578Q-myc-strep (Ritz et al. 2011, NCB)
resistance markers :
335
tags :
Unknown
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA