This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
sgEML4
catalog :
85568
citations: 1
Reference
Guernet A, Mungamuri S, Cartier D, Sachidanandam R, Jayaprakash A, Adriouch S, et al. CRISPR-Barcoding for Intratumor Genetic Heterogeneity Modeling and Functional Analysis of Oncogenic Driver Mutations. Mol Cell. 2016;63:526-38 pubmed publisher
product information
Catalog Number :
85568
Product Name :
sgEML4
article :
doi10.1016/j.molcel.2016.06.017
id22713
pubmed_id27453044
bacterial resistance :
Ampicillin
cloning :
backbonepSpCas9(BB)-2A-Puro (PX459)
backbone_mutationInsertion of a sgRNA sequence targeting EML4
backbone_originFeng Zhang
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
sgRNA sequence from Choi and Meyerson (2014), Nat Commun 5, 3728
growth strain :
Expresses sgRNA targeting human EML4 between exon 13 and 14
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3BbsI
cloning_site_5BbsI
promoter
sequencing_primer_3
sequencing_primer_5GAGGGCCTATTTCCCATGATTCC
site_3_destroyed1
site_5_destroyed1
entrez_gene
aliasesC2orf2, ELP120, EMAP-4, EMAPL4, ROPP120
geneEML4
id27436
genbank_ids
mutation
nameechinoderm microtubule associated protein like 4
shRNA_sequenceGACCTGAACAGCAAGTTTGT
size
species
9606
Homo sapiens
tags
resistance markers :
3155
tags :
Unknown
terms :
Puromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA