This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
sgKRAS(A)
catalog :
85566
citations: 1
Reference
Guernet A, Mungamuri S, Cartier D, Sachidanandam R, Jayaprakash A, Adriouch S, et al. CRISPR-Barcoding for Intratumor Genetic Heterogeneity Modeling and Functional Analysis of Oncogenic Driver Mutations. Mol Cell. 2016;63:526-38 pubmed publisher
product information
Catalog Number :
85566
Product Name :
sgKRAS(A)
article :
doi10.1016/j.molcel.2016.06.017
id22713
pubmed_id27453044
bacterial resistance :
Ampicillin
cloning :
backbonepSpCas9(BB)-2A-Puro (PX459)
backbone_mutationInsertion of a sgRNA sequence targeting KRAS
backbone_originFeng Zhang
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth strain :
Expresses sgRNA targeting human KRAS around codon G12
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3BbsI
cloning_site_5BbsI
promoter
sequencing_primer_3
sequencing_primer_5GAGGGCCTATTTCCCATGATTCC
site_3_destroyed1
site_5_destroyed1
entrez_gene
aliases'C-K-RAS, C-K-RAS, CFC2, K-RAS2A, K-RAS2B, K-RAS4A, K-RAS4B, K-Ras, K-Ras 2, KI-RAS, KRAS1, KRAS2, NS, NS3, OES, RALD, RASK2, c-Ki-ras, c-Ki-ras2
geneKRAS
id3845
genbank_ids
mutation
nameKRAS proto-oncogene, GTPase
shRNA_sequenceGAATATAAACTTGTGGTAGT
size
species
9606
Homo sapiens
tags
resistance markers :
3155
tags :
Unknown
terms :
Puromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA