This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pDule2-3-nitroTyrosine (5B)
catalog :
85499
citations: 1
Reference
Cooley R, Feldman J, Driggers C, Bundy T, Stokes A, Karplus P, et al. Structural basis of improved second-generation 3-nitro-tyrosine tRNA synthetases. Biochemistry. 2014;53:1916-24 pubmed publisher
product information
Catalog Number :
85499
Product Name :
pDule2-3-nitroTyrosine (5B)
article :
doi10.1021/bi5001239
id22700
pubmed_id24611875
bacterial resistance :
Spectinomycin
cloning :
backbonepDule2
backbone_mutation
backbone_origin
backbone_size5400
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth strain :
Plasmid for incorporating the non-canonical amino acid 3-nitroTyrosine with the Mj 3NY (5B) synthetase and cognate amber suppressing tRNA in Ecoli.
origin :
37
pi :
alt_names
3NY (5B) synthetase
3-nitroY (5B) synthetase
3-nitroTyr (5B) synthetase
cloning
clone_methodRestriction Enzyme
cloning_site_3XhoI
cloning_site_5NcoI
promoterlpp (constitutive)
sequencing_primer_3
sequencing_primer_5CGTCACTGCGTCTTTTACTG
site_3_destroyed
site_5_destroyed
entrez_gene
genbank_ids
mutationY32H H70C D158S I159A L162R
name3-nitroTyrosine tRNA synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
shRNA_sequence
size921
species
32644
Other
Methanocaldococcus jannaschii
tags
resistance markers :
3153
tags :
Low Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA