pDule-3-nitroTyrosine (5B) | Addgene 85498 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pDule-3-nitroTyrosine (5B)

catalog :

85498

citations: 3

Reference
Bischoff A, Hamerlynck L, Li A, Roberts T, Ginsberg N, Francis M. Protein-Based Model for Energy Transfer between Photosynthetic Light-Harvesting Complexes Is Constructed Using a Direct Protein-Protein Conjugation Strategy. J Am Chem Soc. 2023;145:15827-15837 pubmed publisher
Porter J, Jang H, Haque M, Stuehr D, Mehl R. Tyrosine nitration on calmodulin enhances calcium-dependent association and activation of nitric-oxide synthase. J Biol Chem. 2020;295:2203-2211 pubmed publisher
Cooley R, Feldman J, Driggers C, Bundy T, Stokes A, Karplus P, et al. Structural basis of improved second-generation 3-nitro-tyrosine tRNA synthetases. Biochemistry. 2014;53:1916-24 pubmed publisher

product information

Catalog Number :

85498

Product Name :

pDule-3-nitroTyrosine (5B)

article :

doi	10.1021/bi5001239
id	22700
pubmed_id	24611875

bacterial resistance :

Tetracycline

cloning :

backbone

pDule

backbone_mutation

backbone_origin

backbone_size

5400

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Bacterial Expression

growth strain :

Plasmid for incorporating the non-canonical amino acid 3-nitroTyrosine with the Mj 3NY (5B) synthetase and cognate amber suppressing tRNA in Ecoli.

origin :

pi :

alt_names

3NY (5B) synthetase

3-nitroY (5B) synthetase

3-nitroTyr (5B) synthetase

cloning

clone_method	Restriction Enzyme
cloning_site_3	XhoI
cloning_site_5	NcoI
promoter	lpp (constitutive)
sequencing_primer_3
sequencing_primer_5	CGTCACTGCGTCTTTTACTG
site_3_destroyed
site_5_destroyed

entrez_gene

genbank_ids

mutation

Y32H H70C D158S I159A L162R

name

3-nitroTyrosine tRNA synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system

shRNA_sequence

size

921

species

32644

Other

Methanocaldococcus jannaschii