This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
mCer-TG2-YFP
catalog :
85471
citations: 1
Reference
Caron N, Munsie L, Keillor J, Truant R. Using FLIM-FRET to measure conformational changes of transglutaminase type 2 in live cells. PLoS ONE. 2012;7:e44159 pubmed publisher
product information
Catalog Number :
85471
Product Name :
mCer-TG2-YFP
article :
doi10.1371/journal.pone.0044159
id22692
pubmed_id22952912
bacterial resistance :
Kanamycin
cloning :
backbonemCerulean-C1
backbone_mutationeYFP insert cloned into BamHI and XbaI sites at the 3' end of the multiple cloning site (MCS).
backbone_origin
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
The hTG2 insert contains V224G and A141T mutations. The depositor states that these mutations do not affect plasmid function.
growth strain :
TG2 biosensor that allows for quantitative assessment of TG2 conformational changes in live cells using FRET
origin :
37
pi :
alt_names
transglutaminase 2
cloning
clone_methodRestriction Enzyme
cloning_site_3Acc65I
cloning_site_5BglII
promoterCMV
sequencing_primer_3EGFP-N
sequencing_primer_5CMV-F
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesG(h), TG(C), TGC, hTG2, tTG
geneTGM2
id7052
genbank_ids
mutation
namehTG2
shRNA_sequence
size
species
9606
Homo sapiens
tags
locationN terminal on backbone
tagmCerulean
locationC terminal on backbone
tagEYFP
resistance markers :
3151
3152
tags :
Unknown
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA