This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
SHP2(T253M/Q257L)@pLKO-Trex-SBP
catalog :
85458
citations: 1
Reference
Chen Y, LaMarche M, Chan H, Fekkes P, García Fortanet J, Acker M, et al. Allosteric inhibition of SHP2 phosphatase inhibits cancers driven by receptor tyrosine kinases. Nature. 2016;535:148-52 pubmed
product information
Catalog Number :
85458
Product Name :
SHP2(T253M/Q257L)@pLKO-Trex-SBP
article :
doi10.1038/nature18621
id22687
pubmed_id27362227
bacterial resistance :
Ampicillin
cloning :
backbonepLKO-trex-SBP-DEST-Neo
backbone_mutation
backbone_origin
backbone_size9600
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Lentiviral
growth strain :
SHP2 mutant gene expression
origin :
37
pi :
alt_names
PTPN11
cloning
clone_methodGateway Cloning
cloning_site_3
cloning_site_5
promoterRSV
sequencing_primer_3TCATCTGAAACTTTTCTGCTGTTGC
sequencing_primer_5ATGACATCGCGGAGATGGTTTCACC
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesBPTP3, CFC, JMML, METCDS, NS1, PTP-1D, PTP2C, SH-PTP2, SH-PTP3, SHP2
genePTPN11
id5781
genbank_ids
mutationT253M/Q257L
nameSHP2
shRNA_sequence
size2200
species
9606
Homo sapiens
tags
locationN terminal on backbone
tagSBP Tag
resistance markers :
3154
tags :
Unknown
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA