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product summary
company name :
Addgene
product type :
cDNA
product name :
pAAV-RSV-SpCas9
catalog :
85450
citations: 5
Reference |
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Duan Y, Ma G, Huang X, D Amore P, Zhang F, Lei H. The Clustered, Regularly Interspaced, Short Palindromic Repeats-associated Endonuclease 9 (CRISPR/Cas9)-created MDM2 T309G Mutation Enhances Vitreous-induced Expression of MDM2 and Proliferation and Survival of Cells. J Biol Chem. 2016;291:16339-47 pubmed publisher
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product information
Catalog Number :
85450
Product Name :
pAAV-RSV-SpCas9
article :
doi | 10.1074/jbc.M116.729467 |
id | 21713 |
pubmed_id | 27246850 |
bacterial resistance :
Ampicillin
cloning :
backbone | pAAV | |
backbone_mutation | ||
backbone_origin | ||
backbone_size | ||
promoter | ||
sequencing_primer_3 | ||
sequencing_primer_5 | ||
vector_types |
|
growth notes :
We replaced the pMecp2 promoter of SpCas9 (Addgene: 60957) with a RSV (Rous Sarcoma Virus) promoter, which was amplified from a lentiviral vector for shRNA-MDM2
growth strain :
Express SpCas9 in mammalian cells
origin :
37
pi :
|
plasmid copy :
We purchased a lentiviral vector with shRNA-MDM2 from GE Dharmacon (Lafayette, CO)
resistance markers :
3146 |
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
Watertown, MA 02472
info@addgene.org
https://www.addgene.org617.225.9000
headquarters: USA
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