This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pAAV-RSV-SpCas9
catalog :
85450
citations: 5
Reference
Han H, Yang Y, Jiao Y, Qi H, Han Z, Wang L, et al. Leverage of nuclease-deficient CasX for preventing pathological angiogenesis. Mol Ther Nucleic Acids. 2023;33:738-748 pubmed publisher
Boender A, Boon M, Albers H, Eck S, Fricker B, Kelly A, et al. An AAV-CRISPR/Cas9 strategy for gene editing across divergent rodent species: Targeting neural oxytocin receptors as a proof of concept. Sci Adv. 2023;9:eadf4950 pubmed publisher
Wu W, Yang Y, Yao F, Dong L, Xia X, Zhang S, et al. AAV-mediated in vivo genome editing in vascular endothelial cells. Methods. 2020;: pubmed publisher
Moghadam F, LeGraw R, Velazquez J, Yeo N, Xu C, Park J, et al. Synthetic immunomodulation with a CRISPR super-repressor in vivo. Nat Cell Biol. 2020;22:1143-1154 pubmed publisher
Duan Y, Ma G, Huang X, D Amore P, Zhang F, Lei H. The Clustered, Regularly Interspaced, Short Palindromic Repeats-associated Endonuclease 9 (CRISPR/Cas9)-created MDM2 T309G Mutation Enhances Vitreous-induced Expression of MDM2 and Proliferation and Survival of Cells. J Biol Chem. 2016;291:16339-47 pubmed publisher
product information
Catalog Number :
85450
Product Name :
pAAV-RSV-SpCas9
article :
doi10.1074/jbc.M116.729467
id21713
pubmed_id27246850
bacterial resistance :
Ampicillin
cloning :
backbonepAAV
backbone_mutation
backbone_origin
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
AAV
growth notes :
We replaced the pMecp2 promoter of SpCas9 (Addgene: 60957) with a RSV (Rous Sarcoma Virus) promoter, which was amplified from a lentiviral vector for shRNA-MDM2
growth strain :
Express SpCas9 in mammalian cells
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3AgeI
cloning_site_5XbaI
promoterpRSV
sequencing_primer_3CGGACCGGTTTTATGTATCGAGCTAGGCAC
sequencing_primer_5CGGTCTAGAaatgtagtcttatgcaatac
site_3_destroyed
site_5_destroyed
entrez_gene
genbank_ids
mutation
namepRSV
shRNA_sequence
size
species
32644
Other
tags
plasmid copy :
We purchased a lentiviral vector with shRNA-MDM2 from GE Dharmacon (Lafayette, CO)
resistance markers :
3146
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA