Dat - EGFP - Tol2 | Addgene 85418 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

Dat - EGFP - Tol2

catalog :

85418

citations: 1

Reference
Xi Y, Yu M, Godoy R, Hatch G, Poitras L, Ekker M. Transgenic zebrafish expressing green fluorescent protein in dopaminergic neurons of the ventral diencephalon. Dev Dyn. 2011;240:2539-47 pubmed publisher

product information

Catalog Number :

85418

Product Name :

Dat - EGFP - Tol2

article :

doi	10.1002/dvdy.22742
id	22671
pubmed_id	21932324

bacterial resistance :

Ampicillin

cloning :

backbone

modified pGEM vector with Tol2 arms

backbone_mutation

backbone_origin

backbone_size

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Other

zebrafish transgenesis

growth notes :

Contains the dat genomic sequences, including approximately 13 kb of its 50-flanking region, but excluding the last coding exon and 30-flanking region. The EGFP coding sequence is cloned in frame into dat exon 1. Due to large size, transformation and DNA prep efficiencies may be low. Electroporation is recommended for transformation and low copy prep protocols using large starting volumes are recommended for purifying the DNA.

growth strain :

for making transgenic zebrafish expressing GFP under the control of cis-regulatory elements of the dopamine transporter (dat) gene

growth temp :

Electroporation is recommended for transformation due to plasmid size.

origin :

pi :

alt_names

dat

cloning

clone_method	Unknown
cloning_site_3
cloning_site_5
promoter	dat
sequencing_primer_3	M13-R
sequencing_primer_5	M13F20
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	da, dat
gene	slc6a3
id	80787

genbank_ids

mutation

name

dopamine transporter

shRNA_sequence

size

27000

species

7955

Danio rerio