HIV-1 Gag-mCherry | Addgene 85390 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

HIV-1 Gag-mCherry

catalog :

85390

citations: 2

Reference
Xu D, Jiang W, Wu L, Gaudet R, Park E, Su M, et al. PLSCR1 is a cell-autonomous defence factor against SARS-CoV-2 infection. Nature. 2023;619:819-827 pubmed publisher
Miyauchi K, Marin M, Melikyan G. Visualization of retrovirus uptake and delivery into acidic endosomes. Biochem J. 2011;434:559-69 pubmed publisher

product information

Catalog Number :

85390

Product Name :

HIV-1 Gag-mCherry

article :

doi	10.1042/BJ20101588
id	22662
pubmed_id	21175427

bacterial resistance :

Ampicillin

cloning :

backbone

pcDNA3.1/Zeo (+)

backbone_mutation

backbone_origin

Invitrogen

backbone_size

5015

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth notes :

Gag fragment was amplified from p96ZM651gag-opt vector [NIH AIDS Research and Reference Reagent Program] and cloned into pcDNA3.1/Zeo(+) using HindIII and BamHI sites. mCherry fragment was amplified from pRSET-BmCherry (from Dr R. Tsien, University of California, San Diego) and cloned into pcDNA3.1/Zeo(+) using BamHI and XhoI sites.

growth strain :

Visualization marker for viral core to measure cellular uptake

origin :

pi :

alt_names

cloning

clone_method	Restriction Enzyme
cloning_site_3	XhoI
cloning_site_5	HindIII
promoter	CMV
sequencing_primer_3	BGH-rev
sequencing_primer_5	CMV-F
site_3_destroyed
site_5_destroyed

entrez_gene

genbank_ids

mutation

name

HIV-1 gag

shRNA_sequence

size

2214

species

32644

Other

Human immunodeficiency virus 1