This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
PH Akt-Venus
catalog :
85223
citations: 1
Reference
O Neill P, Kalyanaraman V, Gautam N. Subcellular optogenetic activation of Cdc42 controls local and distal signaling to drive immune cell migration. Mol Biol Cell. 2016;27:1442-50 pubmed publisher
product information
Catalog Number :
85223
Product Name :
PH Akt-Venus
article :
doi10.1091/mbc.E15-12-0832
id22656
pubmed_id26941336
bacterial resistance :
Ampicillin
cloning :
backbonepcDNA3.1
backbone_mutation
backbone_origin
backbone_size5428
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth strain :
Venus tagged PH domain of Akt for use as a PIP3 sensor
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3XbaI
cloning_site_5EcoR1
promoterCMV
sequencing_primer_3BGH reverse primer
sequencing_primer_5T7 primer
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesAKT, PKB, PKB-ALPHA, PRKBA, RAC, RAC-ALPHA
geneAKT1
id207
genbank_ids
NM_001014431.1
mutation
namePH AKT(1-148)
shRNA_sequence
size444
species
9606
Homo sapiens
tags
locationC terminal on insert
tagVenus
resistance markers :
1218
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA