mTiam1-mcherry-sspB in pcDNA3.1 | Addgene 85221 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

mTiam1-mcherry-sspB in pcDNA3.1

catalog :

85221

citations: 2

Reference
Miao Y, Djeffal Y, De Simone A, Zhu K, Lee J, Lu Z, et al. Reconstruction and deconstruction of human somitogenesis in vitro. Nature. 2023;614:500-508 pubmed publisher
O Neill P, Kalyanaraman V, Gautam N. Subcellular optogenetic activation of Cdc42 controls local and distal signaling to drive immune cell migration. Mol Biol Cell. 2016;27:1442-50 pubmed publisher

product information

Catalog Number :

85221

Product Name :

mTiam1-mcherry-sspB in pcDNA3.1

article :

doi	10.1091/mbc.E15-12-0832
id	22656
pubmed_id	26941336

bacterial resistance :

Ampicillin

cloning :

backbone

pcDNA3.1

backbone_mutation

backbone_origin

invitrogen

backbone_size

5428

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth strain :

Role of Rac selective GEF domain from Tiam1 in immune cell migration

origin :

pi :

alt_names

Tiam1

cloning

clone_method	Restriction Enzyme
cloning_site_3	EcoR1
cloning_site_5	KpnI
promoter	CMV
sequencing_primer_3	BGH reverse primer
sequencing_primer_5	T7 primer
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	AI847750, D16Ium10, D16Ium10e
gene	Tiam1
id	21844

genbank_ids

NP_033410

mutation

name

mTiam1 (1030-1406)

shRNA_sequence

size

species

10090

Mus musculus