pFLAG-CMV2-Hsp27-S15D/S78D/S82D | Addgene 85188 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pFLAG-CMV2-Hsp27-S15D/S78D/S82D

catalog :

85188

citations: 3

Reference
Bonavita R, Scerra G, Di Martino R, Nuzzo S, Polishchuk E, Di Gennaro M, et al. The HSPB1-p62/SQSTM1 functional complex regulates the unconventional secretion and transcellular spreading of the HD-associated mutant huntingtin protein. Hum Mol Genet. 2023;32:2269-2291 pubmed publisher
Gallagher E, Holzbaur E. The selective autophagy adaptor p62/SQSTM1 forms phase condensates regulated by HSP27 that facilitate the clearance of damaged lysosomes via lysophagy. Cell Rep. 2023;42:112037 pubmed publisher
Li J, Fu X, Cao S, Li J, Xing S, Li D, et al. Membrane-associated androgen receptor (AR) potentiates its transcriptional activities by activating heat shock protein 27 (HSP27). J Biol Chem. 2018;293:12719-12729 pubmed publisher

product information

Catalog Number :

85188

Product Name :

pFLAG-CMV2-Hsp27-S15D/S78D/S82D

article :

doi
id	22643
pubmed_id

bacterial resistance :

Ampicillin

cloning :

backbone

pFLAG-CMV-2

backbone_mutation

backbone_origin

backbone_size

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth notes :

The pFLAG-CMV2-Hsp27 wild-type plasmid was obtained from Dr. Park and is described by Park KJ, Gaynor RB, Kwak YT. J Biol Chem 2003; 278:35272-35278. PMID:12829720

growth strain :

Hsp27 expression plasmid

origin :

pi :

alt_names

cloning

clone_method	Unknown
cloning_site_3
cloning_site_5
promoter	CMV
sequencing_primer_3	hGH-pA-R
sequencing_primer_5	CMV-F
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	CMT2F, HEL-S-102, HMN2B, HMND3, HS.76067, HSP27, HSP28, Hsp25, SRP27
gene	HSPB1
id	3315

genbank_ids

mutation

S15D/S78D/S82D, phosphomimicking mutation

name

Hsp27

shRNA_sequence

size

species

9606

Homo sapiens