pLV-CDH5-mRuby3-IRES-puro | Addgene 85144 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pLV-CDH5-mRuby3-IRES-puro

catalog :

85144

citations: 2

Reference
Lyu Q, Xu S, Lyu Y, Choi M, Christie C, Slivano O, et al. SENCR stabilizes vascular endothelial cell adherens junctions through interaction with CKAP4. Proc Natl Acad Sci U S A. 2019;116:546-555 pubmed publisher
Hayer A, Shao L, Chung M, Joubert L, Yang H, Tsai F, et al. Engulfed cadherin fingers are polarized junctional structures between collectively migrating endothelial cells. Nat Cell Biol. 2016;18:1311-1323 pubmed publisher

product information

Catalog Number :

85144

Product Name :

pLV-CDH5-mRuby3-IRES-puro

article :

doi	10.1038/ncb3438
id	22933
pubmed_id	27842057

bacterial resistance :

Ampicillin

cloning :

backbone

pLV-CAG-IRES-Puro

backbone_mutation

backbone_origin

backbone_size

8785

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Lentiviral

growth notes :

Remark on cloning and propagating of cDNA encoding human CDH5: In our experience, CDH5 cDNA was toxic to bacteria when cloned into CMV-driven expression plasmids, resulting in small or mutated clones.

growth strain :

lentiviral expression of human VE-cadherin, C-terminally tagged with mRuby3

origin :

pi :

alt_names

CDH5

cloning

clone_method	Gibson Cloning
cloning_site_3
cloning_site_5
promoter	CAG
sequencing_primer_3	ACACCGGCCTTATTCCAA
sequencing_primer_5	GTTATTGTGCTGTCTCATC
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	7B4, CD144
gene	CDH5
id	1003

genbank_ids

mutation

name

CDH5-mRuby3 (VE-cadherin, C-terminally tagged with mRuby3

shRNA_sequence

size

3114

species

9606

Homo sapiens