This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pLV-CDH5-mCitrine-IRES-puro
catalog :
85143
citations: 2
Reference
Anquetil T, Solinhac R, Jaffre A, Chicanne G, Viaud J, Darcourt J, et al. PI3KC2β inactivation stabilizes VE-cadherin junctions and preserves vascular integrity. EMBO Rep. 2021;22:e51299 pubmed publisher
Hayer A, Shao L, Chung M, Joubert L, Yang H, Tsai F, et al. Engulfed cadherin fingers are polarized junctional structures between collectively migrating endothelial cells. Nat Cell Biol. 2016;18:1311-1323 pubmed publisher
product information
Catalog Number :
85143
Product Name :
pLV-CDH5-mCitrine-IRES-puro
article :
doi10.1038/ncb3438
id22933
pubmed_id27842057
bacterial resistance :
Ampicillin
cloning :
backbonepLV-CAG-IRES-Puro
backbone_mutation
backbone_origin
backbone_size8785
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Lentiviral
growth notes :
Remark on cloning and propagating of cDNA encoding human CDH5: In our experience, CDH5 cDNA was toxic to bacteria when cloned into CMV-driven expression plasmids, resulting in small or mutated clones.
growth strain :
lentiviral expression of human VE-cadherin, C-terminally tagged with mCitrine
origin :
30
pi :
alt_names
CDH5
cloning
clone_methodGibson Cloning
cloning_site_3
cloning_site_5
promoterCAG
sequencing_primer_3ACACCGGCCTTATTCCAA
sequencing_primer_5GTTATTGTGCTGTCTCATC
site_3_destroyed
site_5_destroyed
entrez_gene
aliases7B4, CD144
geneCDH5
id1003
genbank_ids
mutation
nameCDH5-mCitrine (VE-cadherin, C-terminally tagged with mCitrine
shRNA_sequence
size3126
species
9606
Homo sapiens
tags
locationC terminal on insert
tagmCitrine
resistance markers :
336
tags :
High Copy
terms :
Puromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA