This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pCAG-AMPH1-BAR-mCherry
catalog :
85130
citations: 2
Reference
Umapathy A, Torten G, Paniagua A, Chung J, Tomlinson M, Lim C, et al. Spatiotemporal Live-Cell Analysis of Photoreceptor Outer Segment Membrane Ingestion by the Retinal Pigment Epithelium Reveals Actin-Regulated Scission. J Neurosci. 2023;43:2653-2664 pubmed publisher
Hayer A, Shao L, Chung M, Joubert L, Yang H, Tsai F, et al. Engulfed cadherin fingers are polarized junctional structures between collectively migrating endothelial cells. Nat Cell Biol. 2016;18:1311-1323 pubmed publisher
product information
Catalog Number :
85130
Product Name :
pCAG-AMPH1-BAR-mCherry
article :
doi10.1038/ncb3438
id22933
pubmed_id27842057
bacterial resistance :
Ampicillin
cloning :
backbonepCAGEN
backbone_mutation
backbone_origin
backbone_size4798
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
Compatible with 2nd and 3rd generation lentiviral packaging systems.
growth strain :
BAR domain of human Amphiphysin1 (amino acids 1-256), C-terminally tagged with mCherry
origin :
37
pi :
alt_names
AMPH
cloning
clone_methodRestriction Enzyme
cloning_site_3NotI
cloning_site_5EcoRI
promoterCAG
sequencing_primer_3ACACCAGCCACCACCTTC
sequencing_primer_5GTTATTGTGCTGTCTCATC
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesAMPH1
geneAMPH
id273
genbank_ids
NM_001635
mutation
nameBAR domain of human Amphiphysin1 (amino acids 1-256), C-terminally tagged with mCherry
shRNA_sequence
size1494
species
9606
Homo sapiens
tags
locationC terminal on insert
tagmCherry
plasmid copy :
ORFeome v5.1
resistance markers :
336
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA