This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pYES260-sc.eEF2 K509A
catalog :
85120
citations: 1
Reference
Davydova E, Ho A, Malecki J, Moen A, Enserink J, Jakobsson M, et al. Identification and characterization of a novel evolutionarily conserved lysine-specific methyltransferase targeting eukaryotic translation elongation factor 2 (eEF2). J Biol Chem. 2014;289:30499-510 pubmed publisher
product information
Catalog Number :
85120
Product Name :
pYES260-sc.eEF2 K509A
article :
doi10.1074/jbc.M114.601658
id22622
pubmed_id25231979
bacterial resistance :
Ampicillin
cloning :
backbonepYES260
backbone_mutation
backbone_originEuroscarf (Melcher)
backbone_size5933
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Yeast Expression
growth strain :
yeast expression of mutant EEF2
origin :
37
pi :
alt_names
EEF2
cloning
clone_methodRestriction Enzyme
cloning_site_3BamHI
cloning_site_5NcoI
promoterGAL1
sequencing_primer_3CTAATTACATGATGCGGCCCTCTAG
sequencing_primer_5CGGACTACTAGCAGCTGTAATACGACTC
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesYDR385W
geneEFT2
id851993
aliasesYOR133W
geneEFT1
id854301
genbank_ids
mutationK509A
nameEFT1/EFT2
shRNA_sequence
size2529
species
4932
Saccharomyces cerevisiae
tags
locationN terminal on backbone
tag6xHis
resistance markers :
3130
tags :
Low Copy
terms :
URA3
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA