This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pF713-pEGFP-N1-Hs-FL-METTL20 wt
catalog :
85114
citations: 1
Reference
Małecki J, Ho A, Moen A, Dahl H, Falnes P. Human METTL20 is a mitochondrial lysine methyltransferase that targets the β subunit of electron transfer flavoprotein (ETFβ) and modulates its activity. J Biol Chem. 2015;290:423-34 pubmed publisher
product information
Catalog Number :
85114
Product Name :
pF713-pEGFP-N1-Hs-FL-METTL20 wt
article :
doi10.1074/jbc.M114.614115
id22621
pubmed_id25416781
bacterial resistance :
Kanamycin
cloning :
backbonepEGFP-N1
backbone_mutation
backbone_origin
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
Homo sapiens wt METTL20, full-length, cloned into SacI-SacII sites of pEGFP-N1, in frame with EGFP, no STOP codon between METTL20 and EGFP
growth strain :
transfection of HeLa cell for transient expression of human full-length METTL20-EGFP fusion
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3SacII
cloning_site_5SacI
promoterCMV
sequencing_primer_3CGTCGCCGTCCAGCTCGACCAG
sequencing_primer_5CGCAAATGGGCGGTAGGCGTG
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesC12orf72, MGC50559
geneMETTL20
id254013
genbank_ids
mutation
namehuman full-length METTL20
shRNA_sequence
size786
species
9606
Homo sapiens
tags
locationC terminal on backbone
tagEGFP
resistance markers :
3130
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA