This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pF712-pET28a-Hs-delta44-GCDH-NHis wt
catalog :
85113
citations: 1
Reference
Małecki J, Ho A, Moen A, Dahl H, Falnes P. Human METTL20 is a mitochondrial lysine methyltransferase that targets the β subunit of electron transfer flavoprotein (ETFβ) and modulates its activity. J Biol Chem. 2015;290:423-34 pubmed publisher
product information
Catalog Number :
85113
Product Name :
pF712-pET28a-Hs-delta44-GCDH-NHis wt
article :
doi10.1074/jbc.M114.614115
id22621
pubmed_id25416781
bacterial resistance :
Kanamycin
cloning :
backbonepET28a
backbone_mutation
backbone_origin
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth notes :
Homo sapiens wt GCDH, delta 44, cloned into NdeI-NotI sites of pET28a, contains a N-terminal His-tag
growth strain :
expression of recombinant human mature (delta44) GCDH wt with N-terminal 6xHis-tag
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3NotI
cloning_site_5NdeI
promoterT7
sequencing_primer_3TAGAGGCCCCAAGGGGTTATGCTAG
sequencing_primer_5ATGCGTCCGGCGTAGAGG
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesACAD5, GCD
geneGCDH
id2639
genbank_ids
mutationsilent SNP: G1041T
namehuman mature (delta44) GCDH
shRNA_sequence
size1185
species
9606
Homo sapiens
tags
locationN terminal on backbone
tag6xHis
resistance markers :
3130
tags :
Low Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA