This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pTriEx-RhoA-Q63L_mScarlet-i_SGFP2
catalog :
85072
citations: 1
Reference
Bindels D, Haarbosch L, van Weeren L, Postma M, Wiese K, Mastop M, et al. mScarlet: a bright monomeric red fluorescent protein for cellular imaging. Nat Methods. 2017;14:53-56 pubmed publisher
product information
Catalog Number :
85072
Product Name :
pTriEx-RhoA-Q63L_mScarlet-i_SGFP2
article :
doi10.1038/nmeth.4074
id22783
pubmed_id27869816
bacterial resistance :
Ampicillin
cloning :
backbonepTriEx
backbone_mutation
backbone_originNovagen
backbone_size5043
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Bacterial Expression
Insect Expression
growth strain :
Green/red FRET biosensor for RhoA in the GTP locked constitutively active state
origin :
37
pi :
alt_names
RHOA
cloning
clone_methodUnknown
cloning_site_3
cloning_site_5
promoterCMV
sequencing_primer_3TGTGAGCCAGGGCATTG
sequencing_primer_5TAATACGACTCACTATAGGG
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesARH12, ARHA, EDFAOB, RHO12, RHOH12
geneRHOA
id387
genbank_ids
mutationQ63L
nameRhoA Biosensor constitutively active (Q63L)
shRNA_sequence
size2436
species
9606
Homo sapiens
tags
locationN terminal on backbone
tagmScarlet-i and SGFP2
locationC terminal on backbone
tag6xHis
resistance markers :
659
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA