This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
UbcH10 DN AU1
catalog :
8507
citations: 1
Reference
Townsley F, Aristarkhov A, Beck S, Hershko A, Ruderman J. Dominant-negative cyclin-selective ubiquitin carrier protein E2-C/UbcH10 blocks cells in metaphase. Proc Natl Acad Sci U S A. 1997;94:2362-7 pubmed
product information
Catalog Number :
8507
Product Name :
UbcH10 DN AU1
article :
doi10.1073/pnas.94.6.2362
id214
pubmed_id9122200
bacterial resistance :
Ampicillin
cloning :
backbonepJS55
backbone_mutation
backbone_origin
backbone_size4100
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
See author's map. Expression of tagged UbcH10 C114S mutant protein in mammalian cells from early SV40 promoter. Antibodies to AU1 epitope available from BABCO. (Ruderman pJHEAUC-CS2)
origin :
37
pi :
alt_names
ubiquitin-conjugating enzyme H10
E2-C
cloning
clone_methodRestriction Enzyme
cloning_site_3HindIII
cloning_site_5EcoR1
promoter
sequencing_primer_3
sequencing_primer_5T7
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesUBCH10, dJ447F3.2
geneUBE2C
id11065
genbank_ids
mutationC114S
nameUbcH10
shRNA_sequence
size561
species
9606
Homo sapiens
tags
locationC terminal on insert
tagAU1 (DTYRYI)
resistance markers :
429
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA