This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pET-UbcH10-wt
catalog :
8503
citations: 2
Reference
Cl xe9 roux P, Voisin L, Meloche S. Development of a high-throughput assay to identify inhibitors of the ubiquitin-conjugating enzyme UBCH10. SLAS Discov. 2022;27:266-271 pubmed publisher
Bastians H, Topper L, Gorbsky G, Ruderman J. Cell cycle-regulated proteolysis of mitotic target proteins. Mol Biol Cell. 1999;10:3927-41 pubmed
product information
Catalog Number :
8503
Product Name :
pET-UbcH10-wt
article :
doi10.1091/mbc.10.11.3927
id213
pubmed_id10564281
bacterial resistance :
Kanamycin
cloning :
backbonepeT29a
backbone_mutation
backbone_origin
backbone_size5242
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth notes :
See author's map. For bacterial expression of C-terminal His-tagged UbcH10-DN. (Ruderman pET29-DN (C-->S mutation))
origin :
37
pi :
alt_names
ubiquitin-conjugating enzyme H10
cloning
clone_methodRestriction Enzyme
cloning_site_3XhoI
cloning_site_5NdeI
promoter
sequencing_primer_3
sequencing_primer_5T7 Forward
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesUBCH10, dJ447F3.2
geneUBE2C
id11065
genbank_ids
mutation
nameUbcH10
shRNA_sequence
size540
species
9606
Homo sapiens
tags
locationC terminal on backbone
tagHis
resistance markers :
429
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA