This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pAav-MDM2-PQS2-3xHA
catalog :
84886
citations: 1
Reference
Vandemoortele G, Staes A, Gonnelli G, Samyn N, De Sutter D, Vandermarliere E, et al. An extra dimension in protein tagging by quantifying universal proteotypic peptides using targeted proteomics. Sci Rep. 2016;6:27220 pubmed publisher
product information
Catalog Number :
84886
Product Name :
pAav-MDM2-PQS2-3xHA
article :
doi10.1038/srep27220
id22508
pubmed_id27264994
bacterial resistance :
Ampicillin
cloning :
backbonepAav-MCS-PQS2-3xHA
backbone_mutation
backbone_originEyckerman lab
backbone_size4890
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
AAV
growth notes :
Sequencing over ITR sequences may be difficult
growth strain :
rAAV-based template for genome engineering of the MDM2 protein C-terminus containing PQS2 and 3xHA tags and a selection cassette
origin :
37
pi :
alt_names
HDM2
cloning
clone_methodRestriction Enzyme
cloning_site_3EcoRI
cloning_site_5MluI
promoterno
sequencing_primer_3TAGGGCGCGATAACTTCGTA
sequencing_primer_5CCTCTGACTTGAGCGTCGAT
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesACTFS, HDMX, LSKB, hdm2
geneMDM2
id4193
genbank_ids
mutation
nameMDM2 genomic sequence HR1
shRNA_sequence
size1020
species
9606
Homo sapiens
tags
locationC terminal on insert
tagPQS2 3xHA
alt_names
HDM2
cloning
clone_methodRestriction Enzyme
cloning_site_3NheI
cloning_site_5XhoI
promoterno
sequencing_primer_3TACTATGGTTGCTTTGACGT
sequencing_primer_5GGGAGGATTGGGAAGACAAT
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesACTFS, HDMX, LSKB, hdm2
geneMDM2
id4193
genbank_ids
mutation
nameMDM2 genomic insert HR2
shRNA_sequence
size1008
species
9606
Homo sapiens
tags
resistance markers :
2951
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA