pAav-TP53-PQS1-3xFLAG | Addgene 84884 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pAav-TP53-PQS1-3xFLAG

catalog :

84884

citations: 1

Reference
Vandemoortele G, Staes A, Gonnelli G, Samyn N, De Sutter D, Vandermarliere E, et al. An extra dimension in protein tagging by quantifying universal proteotypic peptides using targeted proteomics. Sci Rep. 2016;6:27220 pubmed publisher

product information

Catalog Number :

84884

Product Name :

pAav-TP53-PQS1-3xFLAG

article :

doi	10.1038/srep27220
id	22508
pubmed_id	27264994

bacterial resistance :

Ampicillin

cloning :

backbone

pAav-MCS-PQS1-3xFLAG

backbone_mutation

backbone_origin

Eyckerman lab

backbone_size

4863

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

AAV

growth notes :

This is a targeting vector for modifying the C-terminus of the TP53 gene by adding two tags. A CRE-removable selection cassette is present. The forward sequencing primer needs to sequence over an ITR sequence which can be problematic

growth strain :

rAAV-based template for genome engineering of the p53 C-terminus containing PQS1 and 3xFLAG tags and a selection cassette

origin :

pi :

alt_names

cloning

clone_method	Restriction Enzyme
cloning_site_3	EcoRI
cloning_site_5	MluI
promoter	no
sequencing_primer_3	TAGGGCGCGATAACTTCGTA
sequencing_primer_5	CCTCTGACTTGAGCGTCGAT
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	BCC7, BMFS5, LFS1, P53, TRP53
gene	TP53
id	7157

genbank_ids

mutation

name

TP53 genomic region HR1

shRNA_sequence

size

1090

species

9606

Homo sapiens