This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pCW4
catalog :
8484
citations: 5
Reference
Liu H, Chen C, Liao S, Sohaii D, Cruz C, Burdo T, et al. Strategic self-limiting production of infectious HIV particles by CRISPR in permissive cells. Mol Ther Nucleic Acids. 2023;32:1010-1025 pubmed publisher
Arnan C, Ullrich S, Pulido Quetglas C, Nurtdinov R, Esteban A, Blanco Fernandez J, et al. Paired guide RNA CRISPR-Cas9 screening for protein-coding genes and lncRNAs involved in transdifferentiation of human B-cells to macrophages. BMC Genomics. 2022;23:402 pubmed publisher
Klompe S, Vo P, Halpin Healy T, Sternberg S. Transposon-encoded CRISPR-Cas systems direct RNA-guided DNA integration. Nature. 2019;: pubmed publisher
Aparicio Prat E, Arnan C, Sala I, Bosch N, Guigó R, Johnson R. DECKO: Single-oligo, dual-CRISPR deletion of genomic elements including long non-coding RNAs. BMC Genomics. 2015;16:846 pubmed publisher
Waddell C, Craig N. Tn7 transposition: two transposition pathways directed by five Tn7-encoded genes. Genes Dev. 1988;2:137-49 pubmed
product information
Catalog Number :
8484
Product Name :
pCW4
article :
doi10.1101/gad.2.2.137
id202
pubmed_id2834269
bacterial resistance :
Tetracycline
cloning :
backbonepACYC184
backbone_mutation
backbone_originNEB
backbone_size4245
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth notes :
pCW4 sequence is not complete: the entire plasmid is not known (provided sequence is simply a mosaic of the known pieces). Note: Sequence around 8554 RI site uncertain, there is about 800 bp extra between the first RI site and the adjacent HIII site.
origin :
37
pi :
alt_names
tnsA
tnsB tnsC
tnsD tnsE
cloning
clone_methodRestriction Enzyme
cloning_site_3EcoRI
cloning_site_5EcoRI
promoter
sequencing_primer_3
sequencing_primer_5na
site_3_destroyed1
site_5_destroyed1
entrez_gene
aliasesR721_29
genetnsA
id1238710
genbank_ids
mutation
nameTn7ABCDE
shRNA_sequence
size8552
species
Bacteria
tags
resistance markers :
98
tags :
Low Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA