myc mTOR kinase dead | Addgene 8482 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

myc mTOR kinase dead

catalog :

8482

citations: 4

Reference
Kar A, Lee S, Sahoo P, Thames E, Yoo S, Houle J, et al. MicroRNAs 21 and 199a-3p Regulate Axon Growth Potential through Modulation of Pten and mTor mRNAs. Eneuro. 2021;8: pubmed publisher
Jung J, Macalino S, Cui M, Kim J, Kim H, Song D, et al. Transmembrane 4 L Six Family Member 5 Senses Arginine for mTORC1 Signaling. Cell Metab. 2019;29:1306-1319.e7 pubmed publisher
Rui Y, Xu Z, Chen Z, Zhang S. The GST-BHMT assay reveals a distinct mechanism underlying proteasome inhibition-induced macroautophagy in mammalian cells. Autophagy. 2015;11:812-32 pubmed publisher
Havel J, Li Z, Cheng D, Peng J, Fu H. Nuclear PRAS40 couples the Akt/mTORC1 signaling axis to the RPL11-HDM2-p53 nucleolar stress response pathway. Oncogene. 2015;34:1487-98 pubmed publisher

product information

Catalog Number :

8482

Product Name :

myc mTOR kinase dead

article :

doi
id	201
pubmed_id

bacterial resistance :

Ampicillin

cloning :

backbone

prk5

backbone_mutation

backbone_origin

backbone_size

4716

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth notes :

NOTE: Though the depositors list SalI and NotI as the 5' and 3' cloning sites, respectively, Addgene's quality control sequence shows that there is actually only a SalI at the 3' end of the insert. There is no NotI present, so it may have been destroyed. Please refer to Addgene's sequence for further sub-cloning options.

origin :

pi :

alt_names

cloning

clone_method	Restriction Enzyme
cloning_site_3	not
cloning_site_5	sal
promoter
sequencing_primer_3	SV40pA-R
sequencing_primer_5	SP6
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	Frap1, RAFT1
gene	Mtor
id	56718

genbank_ids

mutation

D2357E V2364I

name

mTOR

shRNA_sequence

size

4500

species

10116

Rattus norvegicus