This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
LCV2 V-3
catalog :
84806
citations: 1
Reference
Yiu G, Tieu E, Nguyen A, Wong B, Smit McBride Z. Genomic Disruption of VEGF-A Expression in Human Retinal Pigment Epithelial Cells Using CRISPR-Cas9 Endonuclease. Invest Ophthalmol Vis Sci. 2016;57:5490-5497 pubmed publisher
product information
Catalog Number :
84806
Product Name :
LCV2 V-3
article :
doi10.1167/iovs.16-20296
id22736
pubmed_id27768202
bacterial resistance :
Ampicillin
cloning :
backboneLentiCRISPRv2
backbone_mutation
backbone_originFeng Zhang lab (Addgene #52961)
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Lentiviral
CRISPR
growth strain :
Cas9 with sgRNA targeting Exon 1 of VEGF-A
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3BsmBI
cloning_site_5BsmBI
promoterU6
sequencing_primer_3
sequencing_primer_5LKO.1 5' (GACTATCATATGCTTACCGT)
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesL-VEGF, MVCD1, VEGF, VPF
geneVEGFA
id7422
genbank_ids
mutation
namegRNA targeting VEGF-A
shRNA_sequenceCGAGCGCCGAGTCGCCACTG
size
species
9606
Homo sapiens
tags
resistance markers :
3119
tags :
Unknown
terms :
Puromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA