This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pLVX-TREtight-Ngn2:2A:Ascl1-PGK-Puro (XTP-N2A)
catalog :
84777
citations: 2
Reference
Mertens J, Herdy J, Traxler L, Schäfer S, Schlachetzki J, Böhnke L, et al. Age-dependent instability of mature neuronal fate in induced neurons from Alzheimer's patients. Cell Stem Cell. 2021;: pubmed publisher
Mertens J, Paquola A, Ku M, Hatch E, Böhnke L, Ladjevardi S, et al. Directly Reprogrammed Human Neurons Retain Aging-Associated Transcriptomic Signatures and Reveal Age-Related Nucleocytoplasmic Defects. Cell Stem Cell. 2015;17:705-718 pubmed publisher
product information
Catalog Number :
84777
Product Name :
pLVX-TREtight-Ngn2:2A:Ascl1-PGK-Puro (XTP-N2A)
article :
doi10.1016/j.stem.2015.09.001
id22548
pubmed_id26456686
bacterial resistance :
Ampicillin
cloning :
backbonePLVX TIGHT PURO
backbone_mutation
backbone_originclonetech
backbone_size7791
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Lentiviral
growth strain :
Second generation lenti expressing Mash1 and Neurogenin for iN transdifferentation to neurons
origin :
37
pi :
alt_names
cloning
clone_methodUnknown
cloning_site_3
cloning_site_5
promoterpTIGHT
sequencing_primer_3CTAAAGCGCATGCTCCAGACTGCC
sequencing_primer_5GCAGAGCTCGTTTAGTG
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesAtoh4, Math4A, NGN2, bHLHa8, ngn-2
geneNEUROG2
id63973
aliasesASH1, HASH1, MASH1, bHLHa46
geneASCL1
id429
genbank_ids
mutation
nameNgn2 and Mash1 with a T2A
shRNA_sequence
size1601
species
9606
Homo sapiens
tags
plasmid copy :
JEROME MERTENS
resistance markers :
154
tags :
High Copy
terms :
Puromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA