This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pGem-sHyper:sAPX
catalog :
84738
citations: 1
Reference
Expósito Rodríguez M, Laissue P, Yvon Durocher G, Smirnoff N, Mullineaux P. Photosynthesis-dependent H2O2 transfer from chloroplasts to nuclei provides a high-light signalling mechanism. Nat Commun. 2017;8:49 pubmed publisher
product information
Catalog Number :
84738
Product Name :
pGem-sHyper:sAPX
article :
doi10.1038/s41467-017-00074-w [pii]
id28189923
pubmed_id28663550
bacterial resistance :
Kanamycin
cloning :
backbonepGemini (Dr. Andrew Simkin)
backbone_mutationbi-directional vector built in house where the expression of two transgenes is driven by back-to-back CaMV35s and Figwort Mosaic Virus (FMV) constitutive promoters.
backbone_originpGWB2
backbone_size16636
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Plant Expression
growth strain :
Simultaneous expresion of stromal sHyPer and stromal Ascorbate peroxidase (APX) in plant cells
origin :
37
pi :
alt_names
Arabidopsis stromal ascorbate peroxidase (At4g08390)
cloning
clone_methodGateway Cloning
cloning_site_3
cloning_site_5
promoterpFMV/p35S
sequencing_primer_3
sequencing_primer_5pFMV34S atcgagcagcagctggcttgtgg/pCaM35S catcgttgaagatgcctctgc
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesAT4G08390, STROMAL ASCORBATE PEROXIDASE, T28D5.80, T28D5_80, stromal ascorbate peroxidase
geneSAPX
id826396
genbank_ids
mutation
namesAPX
shRNA_sequence
size
species
3702
Arabidopsis thaliana
tags
alt_names
cloning
clone_methodGateway Cloning
cloning_site_3
cloning_site_5
promoterpFMV/p35S
sequencing_primer_3
sequencing_primer_5pFMV34S atcgagcagcagctggcttgtgg/pCaM35S catcgttgaagatgcctctgc
site_3_destroyed
site_5_destroyed
entrez_gene
genbank_ids
mutation
namesHyper (HyPer2 targeted to nucleus)
shRNA_sequence
size
species
32644
Other
tags
locationN terminal on insert
tagnuclear localization signals from the coding sequence of Simian Virus 40 (SV40)
locationC terminal on insert
tagnuclear localisation signals from the coding sequence of Simian Virus 40 (SV40)
resistance markers :
3339
tags :
Unknown
terms :
Hygromycin
Other
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA