pGem-nHyPer:cAPX | Addgene 84736 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pGem-nHyPer:cAPX

catalog :

84736

citations: 1

Reference
Expósito Rodríguez M, Laissue P, Yvon Durocher G, Smirnoff N, Mullineaux P. Photosynthesis-dependent H2O2 transfer from chloroplasts to nuclei provides a high-light signalling mechanism. Nat Commun. 2017;8:49 pubmed publisher

product information

Catalog Number :

84736

Product Name :

pGem-nHyPer:cAPX

article :

doi	10.1038/s41467-017-00074-w [pii]
id	28189923
pubmed_id	28663550

bacterial resistance :

Kanamycin

cloning :

backbone

pGemini (Dr. Andrew Simkin)

backbone_mutation

bi-directional vector built in house where the expression of two transgenes is driven by back-to-back CaMV35s and Figwort Mosaic Virus (FMV) constitutive promoters.

backbone_origin

pGWB2

backbone_size

16636

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Plant Expression

growth strain :

Simultaneous expresion of stromal HyPer2 and cytosolic Ascorbate peroxidase (APX) in plant cells

origin :

pi :

alt_names

AT1G07890

cloning

clone_method	Gateway Cloning
cloning_site_3
cloning_site_5
promoter	pFMV/p35S
sequencing_primer_3
sequencing_primer_5	pFMV34S atcgagcagcagctggcttgtgg/pCaM35S catcgttgaagatgcctctgc
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	AT1G07890, ASCORBATE PEROXIDASE, ATAPX01, ATAPX1, CS1, F24B9.2, F24B9_2, MEE6, ascorbate peroxidase 1, maternal effect embryo arrest 6
gene	APX1
id	837304

genbank_ids

mutation

name

APX1

shRNA_sequence

size

753

species

3702

Arabidopsis thaliana