pSLIK CA ROCK2 | Addgene 84649 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pSLIK CA ROCK2

catalog :

84649

citations: 3

Reference
Bose A, Modi K, Dey S, Dalvi S, Nadkarni P, Sudarshan M, et al. 14-3-3γ prevents centrosome duplication by inhibiting NPM1 function. Genes Cells. 2021;26:426-446 pubmed publisher
Kassianidou E, Hughes J, Kumar S. Activation of ROCK and MLCK tunes regional stress fiber formation and mechanics via preferential myosin light chain phosphorylation. Mol Biol Cell. 2017;28:3832-3843 pubmed publisher
Wong S, Ulrich T, Deleyrolle L, MacKay J, Lin J, Martuscello R, et al. Constitutive activation of myosin-dependent contractility sensitizes glioma tumor-initiating cells to mechanical inputs and reduces tissue invasion. Cancer Res. 2015;75:1113-22 pubmed publisher

product information

Catalog Number :

84649

Product Name :

pSLIK CA ROCK2

article :

doi	10.1158/0008-5472.CAN-13-3426
id	18109
pubmed_id	25634210

bacterial resistance :

Ampicillin

cloning :

backbone

pSLIK

backbone_mutation

backbone_origin

Iain Fraser (Addgene plasmid # 25734)

backbone_size

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

Lentiviral

growth notes :

The insert contains Y71C, R77K, F184Y, Y199F, R263K, H274N, R343K, T345N, N372T, M374T, T394V, S407N, AND A411T mutations and premature truncation (ends at amino acid 468) compared to reference sequence (NP001308572.1). The depositor states that these mutations do not affect plasmid function.

growth strain :

Lentiviral expression of doxycycline-inducible constitutively active ROCK2 and co-expression of Venus

origin :

pi :

alt_names

cloning

clone_method	Unknown
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3	hUBCpro-R CTAAGGCCGAGTCTTATGAGCAG
sequencing_primer_5	pTYF-5 GTAGACATAATAGCAACAGAC
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	ROCK-II
gene	ROCK2
id	282041

genbank_ids

mutation

Constitutive Active (please see depositor comments below)

name

ROCK2

shRNA_sequence

size

species

9913

Bos taurus