This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pSLIK DN RhoA
catalog :
84646
citations: 2
Reference
Onken M, Blumer K, Cooper J. Uveal melanoma cells use ameboid and mesenchymal mechanisms of cell motility crossing the endothelium. Mol Biol Cell. 2021;:mbcE20040241 pubmed publisher
MacKay J, Kumar S. Simultaneous and independent tuning of RhoA and Rac1 activity with orthogonally inducible promoters. Integr Biol (Camb). 2014;6:885-94 pubmed publisher
product information
Catalog Number :
84646
Product Name :
pSLIK DN RhoA
article :
doi10.1039/c4ib00099d
id14852
pubmed_id25044255
bacterial resistance :
Ampicillin
cloning :
backbonepSLIK
backbone_mutation
backbone_originIain Fraser (Addgene plasmid # 25734)
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Lentiviral
growth notes :
Insert contains T19N in RhoA insert compared to best match reference sequence (NP_001655.1)
growth strain :
Lentiviral expression of doxycycline-inducible dominant negative RhoA and co-expression of Venus
origin :
37
pi :
alt_names
cloning
clone_methodUnknown
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3hUBCpro-R CTAAGGCCGAGTCTTATGAGCAG
sequencing_primer_5pTYF-5 GTAGACATAATAGCAACAGAC
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesARH12, ARHA, EDFAOB, RHO12, RHOH12
geneRHOA
id387
genbank_ids
mutationDominant Negative
nameRhoA
shRNA_sequence
size
species
9606
Homo sapiens
tags
locationN terminal on insert
tagMyc
resistance markers :
3109
tags :
Unknown
terms :
Other
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA