This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pGEX-HCF-1rep1wt
catalog :
84606
citations: 1
Reference
Capotosti F, Guernier S, Lammers F, Waridel P, Cai Y, Jin J, et al. O-GlcNAc transferase catalyzes site-specific proteolysis of HCF-1. Cell. 2011;144:376-88 pubmed publisher
product information
Catalog Number :
84606
Product Name :
pGEX-HCF-1rep1wt
article :
doi10.1016/j.cell.2010.12.030
id22504
pubmed_id21295698
bacterial resistance :
Ampicillin
cloning :
backbonepGEX-6P-2
backbone_mutationAddition of TEV protease site at 5' end of cloning site and 6x His tag at 3' end of cloning site
backbone_originGE Healthcare
backbone_size5000
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth strain :
Plasmid contains HCF-1rep1wt with GST-tag
origin :
37
pi :
alt_names
HCFC1rep1wt
cloning
clone_methodRestriction Enzyme
cloning_site_3NotI
cloning_site_5BamHI
promoterT7
sequencing_primer_3GEX3-rev
sequencing_primer_5GEX5-for
site_3_destroyed
site_5_destroyed1
entrez_gene
aliasesCFF, HCF, HCF-1, HCF1, HFC1, MAHCX, MRX3, PPP1R89, VCAF, XLID3
geneHCFC1
id3054
genbank_ids
mutation
nameHCF-1rep1wt
shRNA_sequence
size615
species
9606
Homo sapiens
tags
locationN terminal on backbone
tagGST-TEV
locationC terminal on backbone
tagHis
resistance markers :
1955
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA