This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pET30a-SRAP-delCys2
catalog :
84568
citations: 1
Reference
Kweon S, Zhu B, Chen Y, Aravind L, Xu S, Feldman D. Erasure of Tet-Oxidized 5-Methylcytosine by a SRAP Nuclease. Cell Rep. 2017;21:482-494 pubmed publisher
product information
Catalog Number :
84568
Product Name :
pET30a-SRAP-delCys2
article :
doi10.1016/j.celrep.2017.09.055
id28193376
pubmed_id29020633
bacterial resistance :
Kanamycin
cloning :
backbonepET30a
backbone_mutationnone
backbone_originMillipore
backbone_size5400
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth strain :
Encodes murine SRAP domain (250aa) of Hmces/Srap1 with deletion of catalytic Cys2
origin :
37
pi :
alt_names
Hmces
cloning
clone_methodRestriction Enzyme
cloning_site_3XhoI
cloning_site_5BglII
promoterT7
sequencing_primer_3T7 terminator
sequencing_primer_5T7
site_3_destroyed
site_5_destroyed
entrez_gene
aliases8430410A17Rik, C85376
geneHmces
id232210
genbank_ids
mutationdeletion of Cys2
nameSrap1
shRNA_sequence
size750
species
10090
Mus musculus
tags
locationN terminal on backbone
tag6xHis
locationN terminal on backbone
tagS-Tag
locationC terminal on backbone
tag6xHis
resistance markers :
3096
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA