pCLHCX-mPM20D1-flag | Addgene 84566 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pCLHCX-mPM20D1-flag

catalog :

84566

citations: 4

Reference
Wei W, Riley N, Yang A, Kim J, Terrell S, Li V, et al. Cell type-selective secretome profiling in vivo. Nat Chem Biol. 2020;: pubmed publisher
Kim J, Jedrychowski M, Wei W, Fernandez D, Fischer C, Banik S, et al. A Plasma Protein Network Regulates PM20D1 and N-Acyl Amino Acid Bioactivity. Cell Chem Biol. 2020;27:1130-1139.e4 pubmed publisher
Lin H, Long J, Roche A, Svensson K, Dou F, Chang M, et al. Discovery of Hydrolysis-Resistant Isoindoline N-Acyl Amino Acid Analogues that Stimulate Mitochondrial Respiration. J Med Chem. 2018;61:3224-3230 pubmed publisher
Long J, Svensson K, Bateman L, Lin H, Kamenecka T, Lokurkar I, et al. The Secreted Enzyme PM20D1 Regulates Lipidated Amino Acid Uncouplers of Mitochondria. Cell. 2016;166:424-435 pubmed publisher

product information

Catalog Number :

84566

Product Name :

pCLHCX-mPM20D1-flag

article :

doi	10.1016/j.cell.2016.05.071
id	22488
pubmed_id	27374330

bacterial resistance :

Ampicillin

cloning :

backbone

pCLNCX

backbone_mutation

Neo resistance changed to hygro, BamHI before second CMV is destroyed, between HindIII and XhoI is inserted [MluI, BglII, AgeI, BamHI, flag, NotI, 6xHis], WPRE inserted between XhoI and ClaI

backbone_origin

Novus Bio

backbone_size

9400

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

Retroviral

growth strain :

mouse PM20D1, C-term flag and his tags, in mammalian retroviral expression plasmid. Amp/Hygro selection

origin :

pi :

alt_names

PM20D1

cloning

clone_method	Restriction Enzyme
cloning_site_3	MluI
cloning_site_5	HindIII
promoter	CMV
sequencing_primer_3	GGCATTAAAGCAGCGTATCCAC
sequencing_primer_5	GAGCTCGTTTAGTGAACCGTC
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	4732466D17Rik, AI098026
gene	Pm20d1
id	212933

genbank_ids

NM_178079.3

mutation

name

peptidase M20 domain containing 1

shRNA_sequence

size

1600

species

10090

Mus musculus