pAG415-GPDp-EGFP-rap1-K9R | Addgene 84535 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pAG415-GPDp-EGFP-rap1-K9R

catalog :

84535

citations: 1

Reference
Chymkowitch P, NguÃ©a A, Aanes H, Koehler C, Thiede B, Lorenz S, et al. Sumoylation of Rap1 mediates the recruitment of TFIID to promote transcription of ribosomal protein genes. Genome Res. 2015;25:897-906 pubmed publisher

product information

Catalog Number :

84535

Product Name :

pAG415-GPDp-EGFP-rap1-K9R

article :

doi	10.1101/gr.185793.114
id	22475
pubmed_id	25800674

bacterial resistance :

Ampicillin

cloning :

backbone

pAG415

backbone_mutation

backbone_origin

backbone_size

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Yeast Expression

growth notes :

Mutation numbering may differ from listed mutations, but depositor confirms that the correct mutations are present within the plasmid.

growth strain :

This plasmid can be used to express EGFP-tagged Rap1K9R from the GDP promoter in yeast

origin :

pi :

alt_names

cloning

clone_method	Gateway Cloning
cloning_site_3
cloning_site_5
promoter	GPD
sequencing_primer_3	pBluescript-KS
sequencing_primer_5	GPDpro-F
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	YNL216W, GRC4, GRF1, TBA1, TUF1
gene	RAP1
id	855505

genbank_ids

mutation

K(43,61,69,117,240,246,527,582,651)R

name

RAP1

shRNA_sequence

size

species

4932

Saccharomyces cerevisiae