This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pAG415-ADH1p-HA-rap1-K9R
catalog :
84531
citations: 1
Reference
Chymkowitch P, Nguéa A, Aanes H, Koehler C, Thiede B, Lorenz S, et al. Sumoylation of Rap1 mediates the recruitment of TFIID to promote transcription of ribosomal protein genes. Genome Res. 2015;25:897-906 pubmed publisher
product information
Catalog Number :
84531
Product Name :
pAG415-ADH1p-HA-rap1-K9R
article :
doi10.1101/gr.185793.114
id22475
pubmed_id25800674
bacterial resistance :
Ampicillin
cloning :
backbonepAG415
backbone_mutation
backbone_origin
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Yeast Expression
growth notes :
Mutation numbering may differ from listed mutations, but depositor confirms that the correct mutations are present within the plasmid.
growth strain :
This plasmid can be used to express HA-tagged Rap1-K9R from the ADH promoter in yeast
origin :
37
pi :
alt_names
cloning
clone_methodGateway Cloning
cloning_site_3
cloning_site_5
promoterADH1
sequencing_primer_3pBluescript-KS
sequencing_primer_5yADH1-F
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesYNL216W, GRC4, GRF1, TBA1, TUF1
geneRAP1
id855505
genbank_ids
mutationK(43,61,69,117,240,246,527,582,651)R
nameRAP1
shRNA_sequence
size
species
4932
Saccharomyces cerevisiae
tags
locationN terminal on insert
tagHA
resistance markers :
3097
tags :
Unknown
terms :
LEU2
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA