This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
Fuw-dCas9-Dnmt3a
catalog :
84476
citations: 8
Reference
Park H, Shin J, Kim Y, Saito T, Saido T, Kim J. CRISPR/dCas9-Dnmt3a-mediated targeted DNA methylation of APP rescues brain pathology in a mouse model of Alzheimer's disease. Transl Neurodegener. 2022;11:41 pubmed publisher
Sahu S, Agirre E, Inayatullah M, Mahesh A, Tiwari N, Lavin D, et al. A complex epigenome-splicing crosstalk governs epithelial-to-mesenchymal transition in metastasis and brain development. Nat Cell Biol. 2022;24:1265-1277 pubmed publisher
Zhang W, Cao H, Yang J, Zhao J, Liang Z, Kang X, et al. The identification and validation of EphA7 hypermethylation, a novel biomarker, in cervical cancer. BMC Cancer. 2022;22:636 pubmed publisher
Wang C, Leavenworth J, Zhang C, Liu Z, Yuan K, Wang Y, et al. Epigenetic regulation of EIF4A1 through DNA methylation and an oncogenic role of eIF4A1 through BRD2 signaling in prostate cancer. Oncogene. 2022;41:2778-2785 pubmed publisher
Wei Y, Yang C, Zhao Z. Viable offspring derived from single unfertilized mammalian oocytes. Proc Natl Acad Sci U S A. 2022;119:e2115248119 pubmed publisher
Smith J, Banerjee R, Waly R, Urbano A, Gimenez G, Day R, et al. Locus-Specific DNA Methylation Editing in Melanoma Cell Lines Using a CRISPR-Based System. Cancers (Basel). 2021;13: pubmed publisher
Murovec J, Pirc Ž, Yang B. New variants of CRISPR RNA-guided genome editing enzymes. Plant Biotechnol J. 2017;15:917-926 pubmed publisher
Liu X, Wu H, Ji X, Stelzer Y, Wu X, Czauderna S, et al. Editing DNA Methylation in the Mammalian Genome. Cell. 2016;167:233-247.e17 pubmed publisher
product information
Catalog Number :
84476
Product Name :
Fuw-dCas9-Dnmt3a
article :
doi10.1016/j.cell.2016.08.056
id22459
pubmed_id27662091
bacterial resistance :
Ampicillin
cloning :
backboneFUW
backbone_mutation
backbone_origin
backbone_size9207
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Lentiviral
growth strain :
Lentiviral construct to express dCas9 fused with Dnmt3a
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3EcoRI
cloning_site_5AscI
promoter
sequencing_primer_3taaagcagcgtatcc
sequencing_primer_5aactatgcgctcggg
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesDNMT3A2, HESJAS, M.HsaIIIA, TBRS
geneDNMT3A
id1788
genbank_ids
mutation
namedCas9-Dnmt3a
shRNA_sequence
size6933
species
9606
Homo sapiens
100
Synthetic
32644
Other
tags
resistance markers :
227
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA