This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
PPRE-H2B-eGFP
catalog :
84393
citations: 2
Reference
Shoaito H, Chauveau S, Gosseaume C, Bourguet W, Vigouroux C, Vatier C, et al. Peroxisome proliferator-activated receptor gamma-ligand-binding domain mutations associated with familial partial lipodystrophy type 3 disrupt human trophoblast fusion and fibroblast migration. J Cell Mol Med. 2020;24:7660-7669 pubmed publisher
Degrelle S, Shoaito H, Fournier T. New Transcriptional Reporters to Quantify and Monitor PPAR? Activity. PPAR Res. 2017;2017:6139107 pubmed publisher
product information
Catalog Number :
84393
Product Name :
PPRE-H2B-eGFP
article :
doi10.1155/2017/6139107
id22443
pubmed_id29225614
bacterial resistance :
Kanamycin
cloning :
backboneH2B-GFP
backbone_mutationCMV promotor was removed and replaced by PPRE sequence
backbone_origin
backbone_size5100
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth strain :
Fluorescent reporter for PPARgamma activity
origin :
37
pi :
alt_names
PPRE
ApoCIII promotor
cloning
clone_methodRestriction Enzyme
cloning_site_3NheI
cloning_site_5AseI
promoterPPRE
sequencing_primer_3EGFP-N
sequencing_primer_5PPRE-AseI
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesCIMT1, GLM1, NR1C3, PPARG1, PPARG2, PPARG5, PPARgamma
genePPARG
id5468
genbank_ids
mutation
namePPARG response element
shRNA_sequence
size1200
species
9606
Homo sapiens
tags
locationC terminal on backbone
tageGFP
plasmid copy :
Geoff Wahl (Addgene plasmid # 11680)
resistance markers :
3093
3105
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA