This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pTWIN1-His6-Ssp-Httex1-29Q
catalog :
84350
citations: 1
Reference
Vieweg S, Ansaloni A, Wang Z, Warner J, Lashuel H. An Intein-based Strategy for the Production of Tag-free Huntingtin Exon 1 Proteins Enables New Insights into the Polyglutamine Dependence of Httex1 Aggregation and Fibril Formation. J Biol Chem. 2016;291:12074-86 pubmed publisher
product information
Catalog Number :
84350
Product Name :
pTWIN1-His6-Ssp-Httex1-29Q
article :
doi10.1074/jbc.M116.713982
id22431
pubmed_id27002149
bacterial resistance :
Ampicillin
cloning :
backbonepTWIN1
backbone_mutation
backbone_origineBioLabs
backbone_size5800
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth strain :
Expression of the human Huntingtin Exon1 protein containing 29Q in E.coli
origin :
30
pi :
alt_names
Httex1
cloning
clone_methodRestriction Enzyme
cloning_site_3PstI
cloning_site_5NdeI
promoterT7
sequencing_primer_3TGCTAGTTATTGCTCAGCGG
sequencing_primer_5TAATACGACTCACTATAGG
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesHD, IT15, LOMARS
geneHTT
id3064
genbank_ids
mutation
nameHuntingtin Exon 1
shRNA_sequence
size770
species
9606
Homo sapiens
tags
location
tagN-terminal Ssp intein (His-tagged)
resistance markers :
1204
tags :
Low Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA