This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
GUM BUB3
catalog :
84029
citations: 1
Reference
Toledo C, Herman J, Olsen J, Ding Y, Corrin P, Girard E, et al. BuGZ is required for Bub3 stability, Bub1 kinetochore function, and chromosome alignment. Dev Cell. 2014;28:282-94 pubmed publisher
product information
Catalog Number :
84029
Product Name :
GUM BUB3
article :
doi10.1016/j.devcel.2013.12.014
id19765
pubmed_id24462187
bacterial resistance :
Ampicillin and Bleocin (Zeocin)
cloning :
backbonepGIPZ
backbone_mutation
backbone_originOpen Biosystems
backbone_size10381
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Lentiviral
growth notes :
Parental vector (pGIPZ containing a non-silencing control hairpin) was cut with XbaI + NotI to remove the CMV promoter and TurboGFP. UCOE / SFFV enhancer promoter complex and ORF with N-terminal mCherry were PCR amplified and Gibson cloned into the cut vector.
growth strain :
Expression of mCherry tagged BUB3 in human cells
origin :
37
pi :
alt_names
BUB3L
cloning
clone_methodGibson Cloning
cloning_site_3
cloning_site_5
promoterUCOE / SFFV
sequencing_primer_3pCDH-rev
sequencing_primer_5mCherry-F
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesBUB3L, hBUB3
geneBUB3
id9184
genbank_ids
9184
mutation
nameBUB3
shRNA_sequence
size2801
species
9606
Homo sapiens
tags
locationN terminal on insert
tagmCherry
plasmid copy :
BUB3 ORF from Open Biosystems
resistance markers :
3073
tags :
High Copy
terms :
Puromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA