This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pRRL_SRSF2_P95L_mCherry
catalog :
84022
citations: 1
Reference
Kim E, Ilagan J, Liang Y, Daubner G, Lee S, Ramakrishnan A, et al. SRSF2 Mutations Contribute to Myelodysplasia by Mutant-Specific Effects on Exon Recognition. Cancer Cell. 2015;27:617-30 pubmed publisher
product information
Catalog Number :
84022
Product Name :
pRRL_SRSF2_P95L_mCherry
article :
doi10.1016/j.ccell.2015.04.006
id22373
pubmed_id25965569
bacterial resistance :
Ampicillin
cloning :
backbonepRRLSIN.cPPT.PGK-GFP.WPRE
backbone_mutation
backbone_originDidier Trono (Addgene plasmid # 12252)
backbone_size6651
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Lentiviral
growth strain :
expression of SRSF2 mutant in mammalian cells
origin :
37
pi :
alt_names
cloning
clone_methodUnknown
cloning_site_3
cloning_site_5
promoterPGK
sequencing_primer_3TTACTTGTACAGCTCGTCCA
sequencing_primer_5ggggttggggttgcgccttt (hPGK)
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesPR264, SC-35, SC35, SFRS2, SFRS2A, SRp30b
geneSRSF2
id6427
genbank_ids
mutationP95L
nameSRSF2
shRNA_sequence
size1458
species
9606
Homo sapiens
tags
locationC terminal on insert
tagFlag
locationC terminal on insert
tag2TA-mCherry
plasmid copy :
Aravind Ramakrishnan, FHCRC clinical research division
resistance markers :
3071
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA