This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pRKB134_GI-DUX4-intron2(-)
catalog :
83968
citations: 1
Reference
Feng Q, Snider L, Jagannathan S, Tawil R, van der Maarel S, Tapscott S, et al. A feedback loop between nonsense-mediated decay and the retrogene DUX4 in facioscapulohumeral muscular dystrophy. elife. 2015;4: pubmed publisher
product information
Catalog Number :
83968
Product Name :
pRKB134_GI-DUX4-intron2(-)
article :
doi10.7554/eLife.04996
id22368
pubmed_id25564732
bacterial resistance :
Ampicillin
cloning :
backbone -globin NMD reporter wild-type
backbone_mutation
backbone_originLynne Maquat, PMID 9671053
backbone_size6000
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth strain :
to test DUX4 mRNA is subjected to NMD upon intron2 inclusion
origin :
37
pi :
alt_names
cloning
clone_methodGibson Cloning
cloning_site_3
cloning_site_5
promotermouseCMV
sequencing_primer_3AGCATAAACCCTTCTATGACACA
sequencing_primer_5CCTGGCTCACAAGTACCACTAG
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesDUX4L
geneDUX4
id100288687
genbank_ids
FJ439133.1
mutation
nameDUX4-3'UTR-intron2(-)
shRNA_sequence
size597
species
9606
Homo sapiens
tags
resistance markers :
3071
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA